Preparing Mitochondria from Rat Liver Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue because there are no cell walls, and liver in particular is a soft and fairly homogeneous tissue. The metabolism of endotherms requires that some tissues maintain a high density of mitochondria, so the potential yield is high.
Neurophysiology Virtual Lab pilot Neurophysiology is the study of nervous system function. Primarily, it is connected with neurobiology, psychology, neurology, clinical neurophysiology, electrophysiology, biophysical neurophysiology, ethology, neuroanatomy, cognitive science and other brain sciences.
Neuron Simulation Virtual Lab pilot This lab uses a graphical web-based Neuron simulator and models a section of excitable neuronal membrane using the Hodgkin-Huxley equations.
Various experiments will deal with the several parameters of Hodgkin-Huxley equations and will model resting and action potentials, voltage and current clamp, pharmacological effects of drugs that block specific channels etc.
This lab complements some of the exercises in the Virtual Neurophysiology lab. Biochemistry Virtual Lab I Biochemistry is the study of the chemical processes in living organisms. It deals with the structures and functions of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules.
The experiments included in Biochemistry Virtual Lab I are fundamental in nature, dealing with the identification and classification of various carbohydrates, acid-base titrations of amino Isolation of mitochondria, isolation of proteins from their natural sources, etc.
Biochemistry Virtual Lab II Biochemistry Virtual Lab II deals with topics like enzymology, purification of plant pigments and natural products as well as estimation of iodine value and saponification value of fats and oils. Population ecology Virtual Lab I A population is a collection of individuals of the same species that live together in a region.
Population ecology is the study of populations especially population abundance and how they change over time. Crucial to this study are the various interactions between a population and its resources.
Studies on simple models of interacting species is the main focus this simulation oriented lab. Population ecology Virtual Lab II Population ecology is the study of populations especially population abundance and how they change over time.
Studies based on models of predation, competition as seen in interacting species is the main focus this simulation oriented lab. Lab II focuses on applied principles of population ecology for PG students.
Immunology Virtual Lab I The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.
Immunology Virtual Lab II The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.
Microbiology Virtual Lab I The study of microorganisms, which are unicellular or cell-cluster microscopic organisms. This includes eukaryotes such as fungi and, protists and prokaryotes.
Viruses, though not strictly classed as living organisms, are also studied. Microbiology Virtual Lab II To study the biochemical properties of microorganisms, the various techniques employed in cultivation of fungi and viruses along with the molecular level analysis of microbial genome.
This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated.
Cell biology Virtual Lab I Cell biology is an exciting and dynamic area that helps discover the fascinating world of cells. It includes the study of the structure and organization, growth, regulation, movements and interaction of the cells.
Cell biology is closely related to other areas of biology such as genetics, molecular biology, and biochemistry. Cell biology Virtual Lab II Cell biology is an exciting and dynamic area that helps discover the fascinating world of cells.
Bioinformatics Virtual Lab I Bioinformatics is a field which using techniques of informatics to gather, store, analyse and integrate biological data. This virtual lab is an introductory course for undergraduate students and deals with the storage and retrieval of data from different biological databases like Gene, Pubmed, GEO, TAIR, Prosite etc.
Bioinformatics Virtual Lab II This virtual laboratory is for undergraduate and postgraduate students to get a deeper understanding on the analysis of sequence data, its alignment and the evolutionary relationship. The exercises mainly deal with the different algorithms in sequence alignment and provides a computational exploration to the use of various tools used for sequence alignment.
This lab is targeted towards PG students with exercises that will allow one to learn visualising proteins in 3D, how to calculate distance among atoms, find active sites in protein structures and also delve into some structural analysis methods including docking and homology modeling. Combining labs 1, 2 and 3 will give an overall understanding of commonly used computational methods in bioinformatics.Home / General / Isolation of Mitochondria from Cells and Tissues As mitochondria are key to cellular health, it shouldn’t be surprising that an increasing number of human diseases are being traced back to these organelles.
Features of the Mitochondria Isolation Kit for Cultured Cells: • Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest) • Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
Important considerations when performing the isolated mitochondria assessment include consistency in treatment volumes, keeping the mitochondria in MIB and diluting them in EB immediately before the treatments are going to be set up, and using mitochondria within 3 hr of isolation to ensure their ∆Ψ is still being maintained.
The mitochondria can be stored at −80°C for at least 1 yr for some purposes (e.g., protein isolation). Isolation of mitochondria from tissues and cells by differential centrifugation.
Graham JM(1). Author information: (1)Liverpool John Moores University, Liverpool, United Kingdom. Differential centrifugation is used to prepare . Isolation of mitochondria from cultured mammalian cells The preparation of mitochondria using exponentially growing cultured cells is based on the Gaines method (Fig.
2 B) (Enriquez and Attardi, b, Fernandez-Vizarra et al., , Gaines and Attardi, , Gaines, ).